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hanada k 2008 addgene plasmid  (Addgene inc)


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    Addgene inc hanada k 2008 addgene plasmid
    Hanada K 2008 Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hanada+k+2008+addgene+plasmid/pEX-CFP-hLC3WT+(Plasmid+%2324985)/ppr0630481-565-170-172
    Average 91 stars, based on 3 article reviews
    hanada k 2008 addgene plasmid - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Lysis:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Extraction:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Transfection:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Polymerase Chain Reaction:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Mutagenesis:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Mouse Assay:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Recombinant:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Plasmid Preparation:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Software:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Pore Size:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..

    Control:

    Article Title: RAB18 confers protection to lipid droplets from lysolipophagy and counters cellular steatosis
    Article Snippet: .. Tissue-Tek Fischer scientific 4583 Oleic Acid-Albumin from bovine serum Sigma-Aldrich O3008-5ml Palmitic acid Sigma-Aldrich P0500-10G Prequilllan 10mg/ml FATRO NR.08007220401152 PstI-HF NEB R3140S Rat tail Collagen I Roche 11179179001 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific 89900 Rompun 2% Bayer PZN ROTI ® Histofix 4 % Roth P087.5 Smart Cut Buffer NEB B7204S Streptavidin-Magnetic beads Thermo-Fisher Scientific WJ330814 Sucrose Sigma-Aldrich S7903 T4 DNA Ligase NEB M0202S Torin2 Sigma-Aldrich SML1224 Effectene Transfection Reagent Qiagen 301425 Q5 ® High-Fidelity PCR Kit NEB E0555S PierceTM BCA Protein Assay Kit Thermo Fisher Scientific 23250 QuikChange II Agilent 200524 HepG2 ATCC HB 8065 Primary Human Hepatocytes BIOVIT F00995 Oligonucleotides Primers for RAB18 mutation see Table S1: Primers This paper Rab18 siRNA A: Mice Janvier Labs, France C57BL/6NRj Commercial Assays Cell lines and model organism rUrGrGrArUrGrGrArArArUrArArGrGrCrUrArArArCrUr UGC Scrambled non-targeting siRNA duplex: Sense 5’: rCrGrUrUrArArUrCrGrCrGrUrArUrArArUrArCrGrCrG rUAT Antisense5’: rArUrArCrGrCrGrUrArUrUrArUrArCrGrCrGrArUrUrAr ArCrGrArC Recombinant DNA BFP-Sec61b Zurek N, Sparks L, Voeltz G, 2011 Addgene plasmid # 49154 HCMV-RAB18-turbogfp Origene RG205505 pEX-CFP-hLC3WT Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K.,2008 Addgene plasmid # 24985 pFusionRed-C vector Evrogen FP411 pTagGFP2-C vector Evrogen FP191 Software and Algorithms Anaconda Anaconda Inc. https://www.anaconda.com/ ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Inkscape: Open Source Scalable Vector Graphics Editor 1.0 Harrington, B. et al (2004-2005) https://inkscape.org/release/inkscape-1.0/ Jupyter Notebook Project Jupyter https://jupyter.org/about StarDist - Object Detection with Star-convex Shapes Uwe Schmidt, Martin Weiger, Coleman Broaddus and Gene Myers., 2018 https://github.com/mpicbg-csbd/stardist Serial Cloner 2.6 SerialBasics http://serialbasics.free.fr/Serial_Cloner.html Other 6-Well-Plate Sartstedt 83.392 BoltTM 4 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 15-well Invitrogen NW04125BOX Ibidi 8-Well Ibitreat μ-slide Ibidi 80826 Ibidi 8-Well glass bottom μ-slide Ibidi 80827 Ibid 4-Well glass bottom μ-slide Ibidi 80427 Immobilon-P Blotting Sandwich (PVDF membran 0.45μm pore size) Milipore IPSN07852 Origene S3004 Origene SR307888 A Mini Gel Tank Invitrogen A25977 Mini Blot module Invitorgen B100 Mouse control diet Ssniff R/M-H, 10 mm standard diet Ssniff, Soest, Germany Mouse steatogenic diet D16022301 BROGAARDEN Smedevangen 5 350 Lynge, Denmark Supplementary Files This is a list of supplementary les associated with this preprint. ..



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